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TECHNOLOGIES
CELL CULTURE
FLOW CYTOMETRY
CONFOCAL MICROSCOPY
CELL SORTING
CELL TRANSFECTION
TARGETED GENOME EDITING AND GLOBAL GENOME POSITIONING
STABLE MONOCLONAL CELL LINE DEVELOPMENT
PROTEIN EXPRESSION
bacterial, insect, and mammalian hosts
Rosetta - sf9 - HEK – CHO
Transient transfection of mammalian cells
Stable cell line generation for production
PROTEIN PURIFICATION
LC, FPLC, HPLC-based chromatography, gel filtration, various ion-exchange, affinity (His6, GST, Fc), desalting
PROTEIN CHARACTERISATION
SDS/PAGE, WESTERN blotting, ELISA, Analytical FPLC, (CE, CD, MS)
CYTOKINE BIOLOGICAL ACTIVITY
Citokine TF-1 proliferation assay (hIL-4, hGM-CSF)
Citokine K6 proliferation assay (IL-2)
Citokine M1 differentiation assay (LIF)
DC endotoxin sensitisation assay
LAM endotoxin assay
GENE DESIGN AND OPTIMISATION
MOLECULAR CLONING
Plasmid preparation
Cloning into expression vectors
DNA secuencing
qPCR
INVOLVEMENT IN
NGS
ChIP
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Links & Partnes
Links & Partners
Magyar Gazdaságfejlesztési Központ
Center for Clinical Genomics and Personalised medicine
Nuclear Hormon Receptor Research Laboratory - NLAB
Department of Biochemistry and Molecular Biology
Medical and Health Science Center
Research Center for Molecular Medicine
UD-GenoMed Medical Genomic Technologies Ltd
